Merges FASTA of newly designed primers with keeplist primers, accounting for overlapping templates (as long as sequence names are equivalent between the two files).
import multiplex_wormhole as mw
mw.AddKeeplist2FASTA(MAIN_FA, KEEPLIST_FA, OUTPATH=None)
cd ~/multiplex_wormhole #navigate to where mw scripts live
python3 add_keeplist_to_fasta.py -i MAIN_FA -k KEEPLIST_FA [-o OUTPATH]
MAIN_FA (-i) : FASTA of new primers that keeplist will be merged into. Any overlapping sequence names will be removed from this file. KEEPLIST_FA (-k) : FASTA of keeplist primers. OUTPATH (-o) : Filepath to output FASTA. [Default: MAIN_FA prefix + “_plusKeeplist.fa”]